Characterization of Caulobacter Crescentus Ftsz Using Dynamic Light Scattering
نویسندگان
چکیده
Sen Hou, Stefan A. Wieczorek, Tomasz S. Kaminski, Natalia Ziebacz, Marcin Tabaka, Nohemy A. Sorto, Marie H. Foss, Jared T. Shaw, Martin Thanbichler, Douglas B. Weibel, Krzysztof Nieznanski, Robert Holyst, Piotr Garstecki Institute of Physical Chemistry PAS, Kasprzaka 44/52, 01-224 Warsaw, Poland Department of Chemistry, University of California at Davis, 1 Shields Ave, Davis, CA 95616 USA Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA Max Planck Institute for Terrestrial Microbiology, LOEWE Center for Synthetic Microbiology and Faculty of Biology, Philipps University, D-35043 Marburg, Germany Department of Biochemistry, Nencki Institute of Experimental Biology, Pasteur 3, 02-093 Warsaw, Poland Address correspondence to: Piotr Garstecki, Robert Holyst, Institute of Physical Chemistry PAS, Kasprzaka 44/52, 01-224 Warsaw, Poland; E-mail: [email protected] (RH), [email protected] (PG)
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Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2.
FtsZ from most bacteria assembles rapidly in vitro, reaching a steady-state plateau in 5-10 s after addition of GTP. A recent study used a novel dynamic light-scattering technique to assay the assembly of FtsZ from Caulobacter crescentus (CcFtsZ) and reported that assembly required 10 min, ∼100 times slower than for related bacteria. Previous studies had indicated normal, rapid assembly of CcFt...
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